Description
Enhanced GFP Lentivirus (Hygromycin) | 78639-H | Gentaur US, UK & Europe Disrtribition
Category: Cell Signaling/Lentivirus
Application: • Ideal as a positive control for transduction; useful for optimizing transduction assays and to track transduction efficiency over time. • Generation of stable cell line expressing eGFP with hygromycin selection.
Background: N/A
Description: Enhanced green fluorescent protein (eGFP) is a modified (F64L and S65T mutations) version of the native GFP protein isolated from jellyfish (Aequorea victoria), with increased fluorescence and more efficient folding. The Enhanced GFP Lentivirus are replication-incompetent, HIV based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. These viruses constitutively express eGFP under a CMV promoter. eGFP expression and transduction efficiency can easily be verified and optimized via fluorescence microscopy or flow cytometry. eGFP has an excitation wavelength of 488 nm, an emission wavelength of 509 nm, and extinction coefficient of 55,000 M-1cm-1.
Product Type: Lentivirus
Shippement Condition: -80°C
