Description
EL4 Cell Line | CL-0078 | Gentaur US, UK & Europe Disrtribition
Media: CM-0078
Organism: Mus musculus, Mouse
Application: Transfection host.
Background: The cells are resistant to 0. 1mM cortisol and sensitive to 20 μg/mL PHA. A subline(EL4. IL-2) that produces high levels of interleukin-2(IL-2, interleukin 2) is available. A subline(EL4. IL-2) that is resistant to 0. 1mM 5-bromo-2'-deoxyuridine(BUdR) is available. A subline(EL4. BU. 1. OUAr. 1. 1) that is resistant to 0. 1mM 5-bromo-2'-deoxyuridine and 1mM ouabain is available. Tested and found negative for ectromelia virus(mousepox).
Age: N/A
Tissue: Peripheral blood
Morphology: Lymphoblast
Growth Properties: Suspension
Doubling Time: N/A
Biosafty: 1
Medium: DMEM(PM150210)+10% HS+1% P/S(PB180120)
Subculturing: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at the subcultivation ratio of 1:2-1:4.
Ratio: 1:2 to 1:4
Renewal: Every 2 to 3 days
Cryopreservation: Freeze medium: 60% Basal medium+30% FBS+10% DMSOStorage temperature: Liquid nitrogen vapor phase
Culture Conditions: Atmosphere: Air, 95%; CO2, 5%Temperature: 37℃
Effects: N/A
Duration: N/A
Lead Time: 3-5 days
