EL4 Cell Line | CL-0078

EL4 Cell Line | CL-0078 | Gentaur US, UK & Europe Disrtribition

Media: CM-0078

Organism: Mus musculus, Mouse

Application: Transfection host.

Background: The cells are resistant to 0. 1mM cortisol and sensitive to 20 μg/mL PHA. A subline(EL4. IL-2) that produces high levels of interleukin-2(IL-2, interleukin 2) is available. A subline(EL4. IL-2) that is resistant to 0. 1mM 5-bromo-2'-deoxyuridine(BUdR) is available. A subline(EL4. BU. 1. OUAr. 1. 1) that is resistant to 0. 1mM 5-bromo-2'-deoxyuridine and 1mM ouabain is available. Tested and found negative for ectromelia virus(mousepox).

Age: N/A

Tissue: Peripheral blood

Morphology: Lymphoblast

Growth Properties: Suspension

Doubling Time: N/A

Biosafty: 1

Medium: DMEM(PM150210)+10% HS+1% P/S(PB180120)

Subculturing: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at the subcultivation ratio of 1:2-1:4.

Ratio: 1:2 to 1:4

Renewal: Every 2 to 3 days

Cryopreservation: Freeze medium: 60% Basal medium+30% FBS+10% DMSOStorage temperature: Liquid nitrogen vapor phase

Culture Conditions: Atmosphere: Air, 95%; CO2, 5%Temperature: 37℃

Effects: N/A

Duration: N/A

Lead Time: 3-5 days