EGF Receptor / EGFR Antibody | V3981IHC-7ML

EGF Receptor / EGFR Antibody | V3981IHC-7ML | Gentaur US, UK & Europe Disrtribition

Family: Primary antibody

Formulation: Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*

Format: Purified

Clone: GFR/2968R

Host: Rabbit

Clonality: Recombinant Rabbit Monoclonal

Isotype: Rabbit IgG, kappa

Species Reactivity: Human

Application: IHC-P

Application Details: The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Application Note: Titering of the recombinant EGF Receptor antibody may be required for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Purity: Protein A affinity chromatography

Description: Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin. [UniProt]

Immunogen: Recombinant human protein was used as the immunogen for the recombinant EGF Receptor antibody.

Storage: Store the recombinant EGF Receptor antibody at 2-8oC (with azide) or aliquot and store at -20 °C or colder (without azide).

Localization: Cell surface