Description
Ebolavirus EBOV (subtype Zaire, strain Kikwit-95) Envelope Glycoprotein (GP) Protein, His Tag | ZE5-V5221-100ug| Gentaur Distribution US, UK & Europe
EBOV encodes seven structural proteins: nucleoprotein (NP), polymerase cofactor (VP35), (VP40), GP, transcription activator (VP30), VP24, and RNA polymerase (L). GP protein contains 160-kDa envelope-attached glycoprotein (GP) and a 110 kDa secreted glycoprotein (sGP). GP is a class I fusion protein which assembles as trimers on viral surface and plays an important role in virus entry and attachment. Mature GP is a disulfide-linked heterodimer formed by two subunits, GP1 and GP2, which are generated from the proteolytical process of GP precursor (pre-GP) by cellular furin during virus assembly . GP1 is responsible for binding to the receptor (s) on target cells. Interacts with CD209/DC-SIGN and CLEC4M/DC-SIGNR which act as cofactors for virus entry into the host cell. GP2 acts as a class I viral fusion protein. GP1, 2 mediates endothelial cell activation and decreases endothelial barrier function. sGP seems to possess an anti-inflammatory activity as it can reverse the barrier-decreasing effects of TNF alpha.
Source: Ebolavirus EBOV (subtype Zaire, strain Kikwit-95) GP, His Tag (ZE5-V5221) is expressed from human 293 cells (HEK293). It contains AA Ile 33 - Asp 637 (Accession # AAQ55048.1).
Format: Powder.
Tag: C-7×His.
Expression System: HEK293.
Expression Region: Ile 33 - Asp 637.
Conjugate: Unconjugated.
Molecular Weight: 67.2 kDa.
Characteristics: Ebolavirus EBOV (subtype Zaire, strain Kikwit-95) GP, His Tag, containing GP1 and GP2, is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 67.2 kDa. The reducing (R) protein migrates as 21-23 kDa (GP2-delta) and 110-120 kDa (GP1) in SDS-PAGE.
Purity: 85%.
Buffer: PBS, pH7.4.
Storage Conditions: -20℃.
Shipping Conditions: RT
