DNA Polymerase alpha Antibody / POLA1 (N-Terminal Region) | F54189-0.2ML

DNA Polymerase alpha Antibody / POLA1 (N-Terminal Region) | F54189-0.2ML | Gentaur US, UK & Europe Disrtribition

Family: Primary antibody

Formulation: In 1X PBS, pH 7.4, with 0.09% sodium azide

Format: Antigen affinity purified

Clone: N/A

Host Animal: Rabbit

Clonality: Polyclonal (rabbit origin)

Species Reactivity: Human

Application: WB

Buffer: N/A

Limitation: This DNA Polymerase alpha antibody is available for research use only.

Purity: Antigen affinity

Description: Plays an essential role in the initiation of DNA replication. During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1/p180, a regulatory subunit POLA2/p70 and two primase subunits PRIM1/p49 and PRIM2/p58) is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1. The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands. These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively. The reason this transfer occurs is because the polymerase alpha has limited processivity and lacks intrinsic 3' exonuclease activity for proofreading error, and therefore is not well suited for replicating long complexes.

Immunogen: A portion of amino acids 1-33 from human POLA1 was used as the immunogen for the DNA Polymerase alpha antibody.

Storage: Aliquot the DNA Polymerase alpha antibody and store frozen at -20 °C or colder. Avoid repeated freeze-thaw cycles.