Description
Daudi Cell Line | CL-0072 | Gentaur US, UK & Europe Disrtribition
Media: CM-0072
Organism: Homo sapiens, Human
Application: Daudi is a well characterized B lymphoblast cell line which has been employed extensively in studies of mechanisms of leukemogenesis. The cell line is also a suitable transfection host.
Background: The cells are negative for beta-2-microglobulin. They are positive for EBNA, VCA and Surface immunoglobulin(sIg＋).
Age: 16 years
Tissue: Peripheral blood
Morphology: Lymphoblast
Growth Properties: Suspension
Doubling Time: N/A
Biosafty: 2
Medium: RPMI-1640(PM150110)+10% FBS+1% P/S(PB180120)
Subculturing: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at the subcultivation ratio of 1:2-1:4.
Ratio: 1:2 to 1:4
Renewal: Every 2 to 3 days
Cryopreservation: Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture Conditions: Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
Effects: Yes, in agarose; Yes, in nude mice.
Duration: N/A
Lead Time: 3-5 days
