Daudi Cell Line | CL-0072

Daudi Cell Line | CL-0072 | Gentaur US, UK & Europe Disrtribition

Media: CM-0072

Organism: Homo sapiens, Human

Application: Daudi is a well characterized B lymphoblast cell line which has been employed extensively in studies of mechanisms of leukemogenesis. The cell line is also a suitable transfection host.

Background: The cells are negative for beta-2-microglobulin. They are positive for EBNA, VCA and Surface immunoglobulin(sIg＋).

Age: 16 years

Tissue: Peripheral blood

Morphology: Lymphoblast

Growth Properties: Suspension

Doubling Time: N/A

Biosafty: 2

Medium: RPMI-1640(PM150110)+10% FBS+1% P/S(PB180120)

Subculturing: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at the subcultivation ratio of 1:2-1:4.

Ratio: 1:2 to 1:4

Renewal: Every 2 to 3 days

Cryopreservation: Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase

Culture Conditions: Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃

Effects: Yes, in agarose; Yes, in nude mice.

Duration: N/A

Lead Time: 3-5 days