Description
D283 Med Cell Line | CL-0271 | Gentaur US, UK & Europe Disrtribition
Media: CM-0271
Organism: Homo sapiens, Human
Application: N/A
Background: The cells produce tumors in nude mice, and the resulting tumors are positive for expression of neurofibrillary proteins, glutamine synthetase and neuron specific enolase but negative for glial fibrillary acidic proteins and S100 (S-100) protein.
Age: 6 years juvenile
Tissue: Brain/cerebellum, derived from metastatic site: peritoneum
Morphology: Epithelial
Growth Properties: Suspension, multicell aggregates and some adherent cells
Doubling Time: ~52 hours
Biosafty: 1
Medium: MEM (PM150410)+10% FBS+1% P/S (PB180120)
Subculturing: N/A
Ratio: 2×10^5 to 5×10^5cells/mL
Renewal: every 2 to 3 days
Cryopreservation: Freeze Medium: 55% Basal Medium+40% FBS+17% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture Conditions: Atmosphere: Air, 95%; CO2, 5%; Temperature: 37℃
Effects: Yes, in nude mice(The cells form serially transplantable intercranial and subcutaneous tumors.). Yes, in soft agar.
Duration: N/A
Lead Time: 3-5 days
