D283 Med Cell Line | CL-0271

D283 Med Cell Line | CL-0271 | Gentaur US, UK & Europe Disrtribition

Media: CM-0271

Organism: Homo sapiens, Human

Application: N/A

Background: The cells produce tumors in nude mice, and the resulting tumors are positive for expression of neurofibrillary proteins, glutamine synthetase and neuron specific enolase but negative for glial fibrillary acidic proteins and S100 (S-100) protein.

Age: 6 years juvenile

Tissue: Brain/cerebellum, derived from metastatic site: peritoneum

Morphology: Epithelial

Growth Properties: Suspension, multicell aggregates and some adherent cells

Doubling Time: ~52 hours

Biosafty: 1

Medium: MEM (PM150410)+10% FBS+1% P/S (PB180120)

Subculturing: N/A

Ratio: 2×10^5 to 5×10^5cells/mL

Renewal: every 2 to 3 days

Cryopreservation: Freeze Medium: 55% Basal Medium+40% FBS+17% DMSO Storage temperature: Liquid nitrogen vapor phase

Culture Conditions: Atmosphere: Air, 95%; CO2, 5%; Temperature: 37℃

Effects: Yes, in nude mice(The cells form serially transplantable intercranial and subcutaneous tumors.). Yes, in soft agar.

Duration: N/A

Lead Time: 3-5 days