Description
Cytokeratin 8/18 Antibody Cocktail | V8817-20UG | Gentaur US, UK & Europe Disrtribition
Family: Primary antibody
Formulation: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Format: Purified
Clone: SPM192 + SPM265
Host Animal: Mouse
Clonality: Monoclonal (mouse origin)
Species Reactivity: Human
Application: IF, WB, IHC-P
Buffer: N/A
Limitation: This Cytokeratin 8/18 antibody is available for research use only.
Purity: Protein A/G affinity
Description: Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). This MAb cocktail recognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract, and urogenital tract including transitional epithelium. All adenocarcinomas and most squamous carcinomas are positive but keratinizing squamous carcinomas are usually negative. This antibody is useful in demonstrating the presence of Paget cells; there is very little keratin 18 in the normal epidermis so only Paget cells are stained.Immunohistochemical staining with this MAb is indistinguishable from that obtained with monoclonal antibody 5D3.
Immunogen: Keratin preparation from a human carcinoma (SPM192); PMC-42 human breast carcinoma cells (SPM265) was used as the immunogen for the Cytokeratin 8/18 antibody.
Storage: Aliquot the Cytokeratin 8/18 antibody and store frozen at -20 °C or colder. Avoid repeated freeze-thaw cycles.
