Cynomolgus IGF-I R / CD221 Protein, His Tag (MALS verified) | IGR-C5225-100ug

Cynomolgus IGF-I R / CD221 Protein, His Tag (MALS verified) | IGR-C5225-100ug| Gentaur Distribution US, UK & Europe

The Insulin-like Growth Factor 1 Receptor (IGF1) is also known as CD221, JTK13. and is a transmembrane receptor that is activated by IGF-1 and by the related growth factor IGF-2. It belongs to the large class of tyrosine kinase receptors. This receptor mediates the effects of IGF-1, which is a polypeptide protein hormone similar in molecular structure to insulin. IGF1R is make up of two alpha subunits and two beta subunits, the Both the α and β subunits are synthesized from a single mRNA precursor. The precursor is then glycosylated, proteolytically cleaved, and crosslinked by cysteine bonds to form a functional transmembrane αβ chain.The α chains are located extracellularly while the β subunit spans the membrane and are responsible for intracellular signal transduction upon ligand stimulation. IGF1R have a binding site for ATP, which is used to provide the phosphates for autophosphorylation. There is a 60% homology between IGF1R and the insulin receptor. In response to ligand binding, the α chains induce the tyrosine autophosphorylation of the β chains. This event triggers a cascade of intracellular signaling that, while somewhat cell type specific, often promotes cell survival and cell proliferation.

Source: Cynomolgus IGF-I R, His Tag (IGR-C5225) is expressed from human 293 cells (HEK293). It contains AA Glu 31 - Asn 932 (Accession # G7P9I7-1).

Format: Powder.

Tag: C-10×His.

Expression System: HEK293.

Expression Region: Glu 31 - Asn 932.

Conjugate: Unconjugated.

Molecular Weight: 104.9 kDa.

Characteristics: This protein carries a polyhistidine tag at the C-terminus. This protein contains a furin convertase cleavage site, 737-RKRR-740, and will be partially processed into N (α chain) and C-terminal fragment (partial β chain) with calculated MW of 81.1 kDa and 23.8 kDa respectively. The protein migrates as 45-50 kDa (partial β chain), 96-115 kDa (α chain) and 120 kDa (α chain & partial β chain) under reducing (R) condition (SDS-PAGE) due to glycosylation.

Purity: 90%.

Buffer: PBS, pH7.4.

Storage Conditions: -20℃.

Shipping Conditions: RT