Description
Cynomolgus Complement C5 Protein, His Tag | CO5-C52Hx-100ug| Gentaur Distribution US, UK & Europe
Derived from proteolytic degradation of complement C5, C5 anaphylatoxin is a mediator of local inflammatory process. C5 precursor is first processed by the removal of 4 basic residues, forming two chains, beta and alpha, linked by a disulfide bond. C5 convertase activates C5 by cleaving the alpha chain, releasing C5a anaphylatoxin and generating C5b (beta chain + alpha' chain). Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled. The C5a anaphylatoxin interacts with C5AR1 and tick complement inhibitor. C5a is also a potent chemokine which stimulates the locomotion of polymorphonuclear leukocytes and directs their migration toward sites of inflammation.
Source: Cynomolgus Complement C5, His Tag (CO5-C52Hx) is expressed from human 293 cells (HEK293). It contains AA Gln 19 - Cys 1676 (Accession # XP_005580972.1).
Format: Powder.
Tag: C-10×His.
Expression System: HEK293.
Expression Region: Gln 19 - Cys 1676.
Conjugate: Unconjugated.
Molecular Weight: 73.9 kDa (β chain) & 114.9 kDa (α chain).
Characteristics: This protein carries a polyhistidine tag at the C-terminus. The mature form of Complement C5 is a disulfide-linked heterodimer composed of proteolytically cleaved α and β chain. Each α and β chain has a calculated MW of 73.9 kDa (β chain) and 114.9 kDa (α chain). The protein migrates as 70 kDa (β chain) and 116 kDa (α chain) under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity: 95%.
Buffer: PBS, pH7.4.
Storage Conditions: -20℃.
Shipping Conditions: RT
