Description
Cynomolgus CD73 Protein, His Tag (active enzyme) | CD3-C52H9-1mg| Gentaur Distribution US, UK & Europe
5'-nucleotidase (5'-NT), also known as ecto-5'-nucleotidase or CD73 (cluster of differentiation 73), is an enzyme that is encoded by the NT5E gene. CD73 commonly serves to convert AMP to adenosine. Ecto-5-prime-nucleotidase (5-prime-ribonucleotide phosphohydrolase) catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate. Rare allelic variants are associated with a syndrome of adult-onset calcification of joints and arteries (CALJA) affecting the iliac, femoral, and tibial arteries reducing circulation in the legs and the joints of the hands and feet causing pain.
Source: Cynomolgus CD73, His Tag (CD3-C52H9) is expressed from human 293 cells (HEK293). It contains AA Trp 27 - Cys 554 (Accession # EHH53214.1).
Format: Powder.
Tag: C-10×His.
Expression System: HEK293.
Expression Region: Trp 27 - Cys 554.
Conjugate: Unconjugated.
Molecular Weight: 60.0 kDa.
Characteristics: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 60.0 kDa. The protein migrates as 60-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity: 95%.
Buffer: Tris and NaCl, pH7.5.
Storage Conditions: -20℃.
Shipping Conditions: RT
