Cyclin B2 Antibody / CCNB2 | V8196-20UG

Cyclin B2 Antibody / CCNB2 | V8196-20UG | Gentaur US, UK & Europe Disrtribition

Family: Primary antibody

Formulation: 0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Format: Purified

Clone: X29.2

Host Animal: Mouse

Clonality: Monoclonal (mouse origin)

Species Reactivity: Human, Mouse, Rat

Application: ELISA

Buffer: N/A

Limitation: This Cyclin B2 antibody is available for research use only.

Purity: Protein G affinity chromatography

Description: In eukaryotic cells, mitosis is initiated following the activation of a protein kinase known variously as maturation-promoting factor, M phase specific histone kinase or M-phase kinase. This protein kinase is composed of a catalytic subunit (Cdc2), a regulatory subunit (cyclin B) and a low molecular weight subunit (p13-Suc1). The Cdc/cyclin enzyme is subject to multiple levels of control, of which the regulation of the catalytic subunit by tyrosine phosphorylation is the best understood. Tyrosine phosphorylation inhibits the Cdc2/ cyclin B enzyme, and tyrosine dephosphorylation, occurring at the onset of mitosis, directly activates the pre-MPF complex. Evidence has established that B type cyclins not only act on M phase regulatory subunits of the Cdc2 protein kinase, but also activate the Cdc25A and Cdc25B endogenous tyrosine phosphatase, of which Cdc2 is the physiological substrate. The two B type cyclins, cyclin B1 and cyclin B2, have been shown to have distinct tissue distributions.

Immunogen: Recombinant full-length Xenopus laevis Cyclin B2 protein was used as the immunogen for this Cyclin B2 antibody.

Storage: Store the Cyclin B2 antibody at 2-8oC (with azide) or aliquot and store at -20 °C or colder (without azide).