Cyclin B1(V92.1), Biotin conjugate, 0.1mg/mL Antibody | BNCB0680-100

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SKU:
37-BNCB0680-100
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IN STOCK
Weight:
1.00 KGS
€418.00
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Description

Cyclin B1(V92.1), Biotin conjugate, 0.1mg/mL Antibody | BNCB0680-100| Gentaur Distribution US, UK & Europe

Summary.: This antibody recognizes a protein of 55-62 kDa, identified as cyclin B1. In mammals, cyclin B associates with inactive p34cdc2, which facilitates phosphorylation of p34cdc2 at aa 14Thr and 15Tyr. This maintains the inactive state until the end of G2-phase. The inactive cyclin B-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates aa 14Thr and 15Tyr of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when cyclin B is degraded. This degradation process is ubiquitin-dependent and is necessary for the cell to exit mitosis. So, cyclin B-p34cdc2 plays a critical role in G2 to M transition. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors. .

Conjugate: Biotin.

Storage Buffer: PBS, 0.1% BSA, 0.05% azide.

Storage Temperature: 2°C to 8°C.

Application: Flow, intracellular (verified)|IF (verified).

Stability: Stable at room temperature or 37°C (98°F) for 7 days.

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Additional Information

Size:
100 uL
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