CUL4A Polyclonal Antibody | E-AB-19279

CUL4A Polyclonal Antibody | E-AB-19279 | Gentaur US, UK & Europe Disrtribition

Reactivity: Human, Mouse

Host: Rabbit

Isotype: IgG

Application: WB, IHC, ELISA

Research Area: Cancer, Cell Biology

Background: Cullin proteins assemble a large number of RING E3 ubiquitin ligases, participating in the proteolysis through the ubiquitin-proteasome pathway. Two cullin 4 (CUL4) proteins, CUL4A (87 kDa) and CUL4B(104 kDa), have been identified. The two CUL4 sequences are 83% identical. They target certain proteins for degradation by binding protein DDB1 to form a CUL4-DDB1 ubiquitin ligase complex with DDB. They form two individual E3 ligases, DDB1-CUL4ADDB2 and DDB1-CUL4BDDB2 in this process. CUL4A appeared in both the nucleus and the cytosol, suggesting a more complex mechanism for entering the nucleus. CUL4B is localized in the nucleus and facilitates the transfer of DDB1 into the nucleus independently of DDB2.

Lead Time: 7~10 days

Concentration: 1.62 mg/mL

Storage: Store at -20°C. Avoid freeze / thaw cycles.

Immunogen: Synthetic peptide of human CUL4A

Buffer: PBS with 0.05% NaN3 and 40% Glycerol, pH7.4

Purification: Antigen affinity purification

Dilution: WB 1:500-1:2000, IHC 1:50-1:300, ELISA 1:5000-1:10000

Conjugation: Unconjugated

Calculated Molecular Weight: 88 kDa

Observed Molecular Weight: Refer to figures

Clonality: Polyclonal