CT26.WT Cell Line | CL-0071

CT26.WT Cell Line | CL-0071 | Gentaur US, UK & Europe Disrtribition

Media: CM-0071

Organism: Mus musculus, Mouse

Application: The cell line can be used with CT26.CL25 as a model for testing immunotherapy protocols and in studies on the host immune response.

Background: CT26.WT was stably transduced with the retroviral vector LXSN that contains the lacZ gene encoding the model tumor associated antigen(TAA), beta-galactosidase(beta-gal) to obtain the lethal subclone CT26.CL25. The growth rate and lethality of CT26.CL25 and CT26.WT is virtually identical despite the expression by CT26.CL25 of the model TAA, beta-galactosidase, in normal mice. A culture submitted to the ATCC in July of 2001 was found to be contaminated with mycoplasma. Progeny were cured by a 21-day treatment with BM Cycline. The cells were assayed for mycoplasma, by the Hoechst stain, PCR and the standard culture test, after a six-week period following treatment. All tests were negative.

Age: N/A

Tissue: Colon

Morphology: Fibroblast

Growth Properties: Adherent

Doubling Time: N/A

Biosafty: 1

Medium: RPMI-1640(PM150110)+10% FBS+1% P/S(PB180120)

Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.

Ratio: 1:2 to 1:4

Renewal: Every 2 to 3 days

Cryopreservation: Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase

Culture Conditions: Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃

Effects: Yes, in BALB/c mice. Mice inoculated, subcutaneously, developed lethal tumors at 80% frequency with 10^3 cells and at 100% with 10^4 cells. Pulmonary metastases developed when mice were inoculated, intravenously, with 10^4 cells.

Duration: N/A

Lead Time: 3-5 days