CRISPRa (SAM) Jurkat Cell Pool | 78080

CRISPRa (SAM) Jurkat Cell Pool | 78080 | Gentaur US, UK & Europe Disrtribition

Category: CRISPR/Cells

Application: 1. Quickly generating CRISPR-activated cell pools or cell lines in Jurkat cells.
2. Implementing sgRNA CRISPRa screens in Jurkat cells.

Background: N/A

Description: This cell line has been engineered for use with the CRISPR Synergistic Activation Mediator (SAM) system to induce transcriptional activation and expression of any gene of interest. Cells stably express a mutated dCas9 (Streptococcus pyogenes CRISPR associated protein 9), lacking any endonuclease activity, fused to VP64, a transcriptional activator. Stable dCas9-VP64 expression is maintained with Blasticidin resistance. Cells also stably express P65 (Transcription Factor p65, or Nuclear Factor NF-κB p65) and HSF1 (Heat Shock Factor 1) fused with an MS2 tag, which is maintained with Hygromycin resistance. When these cells are transfected with an MS2-tagged sgRNA targeting the promoter region of the gene of interest, dCas9-VP64 and MS2-P65-HSF1 are recruited to the site in the genomic DNA and begin transcription, inducing expression of the desired gene.

Product Type: Cell Pool

Shippement Condition: -80°C