CRISPR-Cas9 Antibody | RQ4684

CRISPR-Cas9 Antibody | RQ4684 | Gentaur US, UK & Europe Disrtribition

Family: Primary antibody

Formulation: Antibody in PBS with 0.02% sodium azide and 50% glycerol

Format: Purified

Clone: ABCH-3

Host Animal: Rabbit

Clonality: Rabbit Monoclonal

Species Reactivity: Streptococcus pyogenes

Application: WB

Buffer: N/A

Limitation: This CRISPR-Cas9 antibody is available for research use only.

Purity: Affinity purified

Description: CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer; Cas9 is inactive in the absence of the 2 guide RNAs (gRNA). Cas9 recognizes the protospacer adjacent motif (PAM) in the CRISPR repeat sequences to help distinguish self versus nonself, as targets within the bacterial CRISPR locus do not have PAMs. PAM recognition is also required for catalytic activity. [UniProt]

Immunogen: A synthetic peptide specific to CRISPR-Cas9 was used as the immunogen for the CRISPR-Cas9 antibody.

Storage: Store the CRISPR-Cas9 antibody at -20 °C.