COS-7 Cell Line | CL-0069

COS-7 Cell Line | CL-0069 | Gentaur US, UK & Europe Disrtribition

Media: CM-0069

Organism: Cercopithecus aethiops

Application: This is an African green monkey kidney fibroblast-like cell line suitable for transfection by vectors requiring expression of SV40 T antigen.

Background: This line contains T antigen, retains complete permissiveness for lytic growth of SV40, supports the replication of ts A209 virus at 40℃, and supports the replication of pure populations of SV40 mutants with deletions in the early region. The line was derived from the CV-1 cell line by transformation with an origin defective mutant of SV40 which codes for wild type T antigen.

Age: N/A

Tissue: Kidney

Morphology: Fibroblast

Growth Properties: Adherent

Doubling Time: N/A

Biosafty: 2

Medium: DMEM(PM150210)+10% FBS+1% P/S(PB180120)

Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.

Ratio: 1:2 to 1:4

Renewal: Every 2 to 3 days

Cryopreservation: Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase

Culture Conditions: Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃

Effects: N/A

Duration: N/A

Lead Time: 3-5 days