Description
Clostridium difficile Toxin A Antibody | V8285-20UG | Gentaur US, UK & Europe Disrtribition
Family: Primary antibody
Formulation: 0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Format: Purified
Clone: 253/422
Host Animal: Mouse
Clonality: Monoclonal (mouse origin)
Species Reactivity: Clostridium difficile
Application: ELISA, IF
Buffer: N/A
Limitation: This Clostridium difficile Toxin A antibody is available for research use only.
Purity: Protein G affinity chromatography
Description: This mAb reacts with C. difficile Toxin A, but not with Cholera subunit a, Cholera toxin, Pseudomonas aeruginosa exotoxin A, H-LT, P-LT. Clostridium difficile is a major nosocomial pathogen that causes antibiotic- associated colitis. Clostridium difficile mediates inflammatory diarrhea by releasing two large protein enterotoxins (Toxin A and Toxin B) that are able to disrupt intestinal epithelial cells via their transferase activity and ability to monoglucosylate members of the Rho family. Clostridium difficile Toxin A is a toxin that is composed of 39 repeats that are responsible for binding to intestinal epithelial cell surface carbohydrates. Clostridium difficile Toxin A causes significant apoptosis of colonocytes which contributes to the formation of ulcers and pseudo-membranes in a pathway that involves p38-dependent activation of p53 and induction of p21, leading to cytochrome c release and caspase-3 activation through Bak activation.
Immunogen: C. difficile Toxin A was used as the immunogen for the Clostridium difficile Toxin A antibody.
Storage: Store the Clostridium difficile Toxin A antibody at 2-8oC (with azide) or aliquot and store at -20 °C or colder (without azide).
