Cleaved PARP1 Antibody | RQ5289

Cleaved PARP1 Antibody | RQ5289 | Gentaur US, UK & Europe Disrtribition

Family: Primary antibody

Formulation: Antibody in PBS with 0.02% sodium azide and 50% glycerol

Format: Purified

Clone: BHH-16

Host Animal: Rabbit

Clonality: Rabbit Monoclonal

Species Reactivity: Human

Application: WB

Buffer: N/A

Limitation: This Cleaved PARP antibody is available for research use only.

Purity: Affinity purified

Description: The main role of PARP (found in the cell nucleus) is to detect and initiate an immediate cellular response to metabolic, chemical, or radiation-induced single-strand DNA breaks (SSB) by signaling the enzymatic machinery involved in the SSB repair. [Wiki] Cleavage of PARP, by enzymes such as caspases or cathepsins, typically inactivates PARP. While in vitro cleavage by caspase occurs throughout the caspase family, preliminary data suggest that caspase-3 and caspase-7 are responsible for in vivo cleavage. Cleavage occurs at aspartic acid 214 and glycine 215, separating PARP into a 24kDA and 89kDA segment. The smaller moiety includes the zinc finger motif requisite in DNA binding. The 89 kDa fragment includes the auto-modification domain and catalytic domain. [Wiki]

Immunogen: A synthetic peptide specific to human PARP (89kDa cleavage segment) was used as the immunogen for the Cleaved PARP antibody.

Storage: Store the Cleaved PARP antibody at -20 °C.