Description
Cleaved PARP1 Antibody | RQ5288 | Gentaur US, UK & Europe Disrtribition
Family: Primary antibody
Formulation: Antibody in PBS with 0.02% sodium azide and 50% glycerol
Format: Purified
Clone: HI-16
Host Animal: Rabbit
Clonality: Rabbit Monoclonal
Species Reactivity: Human, Mouse
Application: IHC-P, WB
Buffer: N/A
Limitation: This Cleaved PARP antibody is available for research use only.
Purity: Affinity purified
Description: The main role of PARP (found in the cell nucleus) is to detect and initiate an immediate cellular response to metabolic, chemical, or radiation-induced single-strand DNA breaks (SSB) by signaling the enzymatic machinery involved in the SSB repair. [Wiki] Cleavage of PARP, by enzymes such as caspases or cathepsins, typically inactivates PARP. While in vitro cleavage by caspase occurs throughout the caspase family, preliminary data suggest that caspase-3 and caspase-7 are responsible for in vivo cleavage. Cleavage occurs at aspartic acid 214 and glycine 215, separating PARP into a 24kDA and 89kDA segment. The smaller moiety includes the zinc finger motif requisite in DNA binding. The 89 kDa fragment includes the auto-modification domain and catalytic domain. [Wiki]
Immunogen: A synthetic peptide specific to human PARP (24kDa cleavage segment) was used as the immunogen for the Cleaved PARP antibody.
Storage: Store the Cleaved PARP antibody at -20 °C.
