Cleaved-CASP8 (D384) Polyclonal Antibody | E-AB-30009

Cleaved-CASP8 (D384) Polyclonal Antibody | E-AB-30009 | Gentaur US, UK & Europe Disrtribition

Reactivity: Human

Host: Rabbit

Isotype: IgG

Application: WB, IHC-p, IF, ELISA

Research Area: Cancer, Cell Biology, Metabolism

Background: Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-AMC. Likely target for the cowpox virus CRMA death inhibitory protein. Isoform 5, isoform 6, isoform 7 and isoform 8 lack the catalytic site and may interfere with the pro-apoptotic activity of the complex.

Lead Time: 7~10 days

Concentration: 1 mg/mL

Storage: Store at -20°C. Avoid freeze / thaw cycles.

Immunogen: Synthesized peptide derived from the C-terminal region of human Caspase-8

Buffer: PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH7.4

Purification: Affinity purification

Dilution: WB 1:500-2000, IHC 1:50-300, IF 1:50-300

Conjugation: Unconjugated

Calculated Molecular Weight: 55 kDa

Observed Molecular Weight: 47+55 kDa

Clonality: Polyclonal