Description
Cleaved-CASP8 (D384) Polyclonal Antibody | E-AB-30009 | Gentaur US, UK & Europe Disrtribition
Reactivity: Human
Host: Rabbit
Isotype: IgG
Application: WB, IHC-p, IF, ELISA
Research Area: Cancer, Cell Biology, Metabolism
Background: Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-AMC. Likely target for the cowpox virus CRMA death inhibitory protein. Isoform 5, isoform 6, isoform 7 and isoform 8 lack the catalytic site and may interfere with the pro-apoptotic activity of the complex.
Lead Time: 7~10 days
Concentration: 1 mg/mL
Storage: Store at -20°C. Avoid freeze / thaw cycles.
Immunogen: Synthesized peptide derived from the C-terminal region of human Caspase-8
Buffer: PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH7.4
Purification: Affinity purification
Dilution: WB 1:500-2000, IHC 1:50-300, IF 1:50-300
Conjugation: Unconjugated
Calculated Molecular Weight: 55 kDa
Observed Molecular Weight: 47+55 kDa
Clonality: Polyclonal
