Description
CFPAC-1 Cell Line | CL-0059 | Gentaur US, UK & Europe Disrtribition
Media: CM-0059
Organism: Homo sapiens, Human
Application: Transfection host.
Background: The cells exhibit ion transport activities consistent with cystic fibrosis and express the product of the CF gene(cystic fibrosis transmembrane regulator, CFTR).CFPAC-1 cells show no effect of cAMP agonists, adenyl cyclase stimulators or phosphodiesterase inhibitors on Cl- flux, but do respond to Ca＋ionophores with increase Cl- efflux.The cells have the most common form of the CF mutation, deletion of three nucleotides resulting in the absence of phenylalanine at position 508.CFPAC-1 cells have epithelial morphology and polarization with apical microvilli, tight junctions and gap junctions.
Age: 26 years
Tissue: Pancreas, derived from metastatic: liver
Morphology: Epithelial
Growth Properties: Adherent
Doubling Time: N/A
Biosafty: 1
Medium: IMDM(PM150510)+10% FBS+1% P/S(PB180120)
Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Ratio: 1:2 to 1:4
Renewal: Every 2 to 3 days
Cryopreservation: Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture Conditions: Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
Effects: Yes, in nude mice.
Duration: N/A
Lead Time: 3-5 days
