Description
CDX2 Antibody | V3964-20UG | Gentaur US, UK & Europe Disrtribition
Family: Primary antibody
Formulation: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Format: Purified
Clone: CDX2/2951R
Host: Rabbit
Clonality: Recombinant Rabbit Monoclonal
Isotype: Rabbit IgG, kappa
Species Reactivity: Human
Application: ELISA, IHC-P
Application Details: ELISA: 2-4ug/ml (order BSA/azide-free format),Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Note: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the recombinant CDX2 antibody to be titered up or down for optimal performance.
1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Purity: Protein A affinity chromatography
Description: The intestine-specific transcription factors CDX1 and CDX2 are important for directing intestinal development, differentiation, proliferation and maintenance of the intestinal phenotype. CDX2 protein expression has been seen in GI carcinomas. Anti-CDX2 has been useful to establish GI origin of metastatic adenocarcinomas and carcinoids and is especially useful to distinguish metastatic colorectal adenocarcinoma from lung adenocarcinoma. However, mucinous carcinomas of the ovary also express CDX2 protein. It limits the usefulness of this marker in the distinction of metastatic colorectal adenocarcinoma from mucinous carcinoma of the ovary.
Immunogen: Amino acids 150-249 from the human protein were used as the immunogen for this recombinant CDX2 antibody.
Storage: Store the recombinant CDX2 antibody at 2-8oC (with azide) or aliquot and store at -20 °C or colder (without azide).
Localization: Nuclear
