Description
CD81 Antibody | V8525-20UG | Gentaur US, UK & Europe Disrtribition
Family: Primary antibody
Formulation: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Format: Purified
Clone: rC81/3442
Host: Mouse
Clonality: Recombinant Mouse Monoclonal
Isotype: Mouse IgG1, kappa
Species Reactivity: Human
Application: Functional Studies, FACS, IF, WB, IHC-P
Application Details: Functional Studies: order Ab format without BSA/sodium azide,Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 0.5-1ug/ml,Western blot: 1-2ug/ml for 2 hours at RT,Immunohistochemistry (FFPE): 2-4ug/ml for 30 minutes at RT
Application Note: Optimal dilution of the recombinant CD81 antibody should be determined by the researcher.
Purity: Protein G affinity chromatography
Description: Recognizes a protein of 26kDa, identified as CD81 (Workshop VI; Code CD81.1). CD81 has a very broad cellular distribution, being expressed on T- and B-lymphocytes, NK cells, thymocytes, eosinophils, fibroblasts, epithelial and endothelial cells. Neutrophils, erythrocytes and platelets are negative, while monocytes are variably positive. CD81 is a member of a family of tetraspans transmembrane proteins, including CD9, CD37, CD53, CD63, and CD82. It associates with CD19, CD21, Leu 13, and integrins on cell membrane and is involved in signal transduction in B lymphocyte development and cell adhesion. CD81 also acts as a receptor for the envelope protein E2 of chronic hepatitis C virus. Antibodies to CD81 have anti-proliferative effects on different lymphoid cell lines, particularly those derived from large cell lymphomas.
Immunogen: Recombinant full-length human CD81 protein was used as the immunogen for the recombinant CD81 antibody.
Storage: Store the recombinant CD811 antibody at 2-8oC (with azide) or aliquot and store at -20 °C or colder (without azide).
Localization: Cytoplasmic, membranous
