Description
CD45RA Antibody (Leukocyte marker) | V8403-100UG | Gentaur US, UK & Europe Disrtribition
Family: Primary antibody
Formulation: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Format: Purified
Clone: rPTPRC/1131
Host: Mouse
Clonality: Recombinant Mouse Monoclonal
Isotype: Mouse IgG1, kappa
Species Reactivity: Human
Application: WB, IHC-P
Application Details: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
Application Note: Optimal dilution of the recombinant CD45RA antibody should be determined by the researcher.
Purity: Protein G affinity chromatography
Description: Recognizes a protein of 205kDa-220kDa, identified as CD45RA. CD45RA is isoforms of the human leukocyte common antigen (CD45). Human CD45 contains three exons which encode peptide segments designated A, B and C, respectively. The differential splicing of the exons generates at least five isoforms, ABC, AB, BC, B and O. This antibody reacts with ABC and BC isoforms. CD45RA is expressed on 40-50% of peripheral CD4+ T-cells, 50% of peripheral CD8+ T-cells, B-cells, and leukemic B-cell lines. T-cells expressing CD45RA are naive or virgin T-cells. T-cells expressing CD45RO are memory T-cells. CD45RA and CD45RO define complementary, predominantly non-overlapping populations of resting peripheral T-cells. This MAb is useful in study on the subpopulation of CD4+ or CD8+ T-cells. It can especially be used to differentiate T-cell lymphomas (CD45RO +ve) from B cell lymphomas (CD45RA +ve).
Immunogen: Recombinant full-length human protein was used as the immunogen for the recombinant CD45RA antibody.
Storage: Store the recombinant CD45RA antibody at 2-8oC (with azide) or aliquot and store at -20 °C or colder (without azide).
Localization: Cell surface
