Description
CD44v4 Antibody | V3688-20UG | Gentaur US, UK & Europe Disrtribition
Family: Primary antibody
Formulation: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Format: Purified
Clone: CDLA44v4-1R
Host: Rabbit
Clonality: Recombinant Rabbit Monoclonal
Isotype: Rabbit IgG, kappa
Species Reactivity: Human
Application: IHC-P
Application Details: Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT,Prediluted IHC only format : incubate for 30 min at RT (1)
Application Note: The stated application concentrations are suggested starting points. Titration of the recombinant CD44v4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Purity: Protein A affinity chromatography
Description: This antibody recognizes an epitope encoded by exon v4 on the variant portion of human CD44. The CD44 molecule belongs to a family of cellular adhesion molecules found on a wide range of normal and malignant cells in epithelial, mesothelial and hemopoiesis tissues. It is a single gene with 20 exons, of which 10 are normally expressed to encode the basic CD44 (H-CAM) molecule. The additional 10 exons (v1 to v10) are only expressed by alternative splicing of the nuclear RNA. The expression of specific cell adhesion molecule CD44 splice variants has been reported to be associated with metastasis in certain human malignancies.
Immunogen: An amino acid sequence from the variant 4 domain of CD44 was used as the immunogen for the recombinant CD44v4 antibody.
Storage: Store the recombinant CD44v4 antibody at 2-8oC (with azide) or aliquot and store at -20 °C or colder (without azide).
Localization: Cell surface, cytoplasmic
