CD44 Antibody / HCAM | V8251SAF-100UG

CD44 Antibody / HCAM | V8251SAF-100UG | Gentaur US, UK & Europe Disrtribition

Family: Primary antibody

Formulation: 1 mg/ml in 1X PBS; BSA free, sodium azide free

Format: Purified

Clone: rHCAM/918

Host: Mouse

Clonality: Recombinant Mouse Monoclonal

Isotype: Mouse IgG1, kappa

Species Reactivity: Human

Application: WB, FACS, IF, IHC-P

Application Details: Western blot: 1-2ug/ml,Flow cytometry: 1-2ug/million cells in 0.1ml,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml

Application Note: Optimal dilution of the antibody should be determined by the researcher.

Purity: Protein G affinity chromatography

Description: Recognizes a cell surface glycoprotein of 80-95kDa (CD44) on lymphocytes, monocytes, and granulocytes (Leucocyte Typing Workshop V). Its epitope is resistant to digestion by trypsin and chymotrypsin. This MAb selectively interferes with lymphocyte binding to lymph node, mucosal and synovial endothelium. The CD44 family of glycoproteins exists in a number of variant isoforms, the most common being the standard 85-95kDa or hematopoietic variant (CD44s). Higher molecular weight isoforms are described in epithelial cells (CD44v), which are believed to function in intercellular adhesion and stromal binding. CD44 immunostaining is commonly used for the discrimination of urothelial transitional cell carcinoma in-situ from non-neoplastic changes in the urothelium.

Immunogen: A recombinant full-length human CD44 protein was used as the immunogen for this recombinant CD44 antibody.

Storage: Store the recombinant CD44 antibody at 2-8oC (with azide) or aliquot and store at -20 °C or colder (without azide).

Localization: N/A