CD32 Antibody / FCGR2A | V2496-20UG

CD32 Antibody / FCGR2A | V2496-20UG | Gentaur US, UK & Europe Disrtribition

Family: Primary antibody

Formulation: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Format: Purified

Clone: 8.7

Host Animal: Mouse

Clonality: Monoclonal (mouse origin)

Species Reactivity: Human

Application: Functional studies, IF

Buffer: N/A

Limitation: This CD32 antibody is available for research use only.

Purity: Protein G affinity chromatography

Description: This mAb reacts with a CD32 (FcgRII) epitope distinct from that defined by mAb 8.26 and the epitope overlaps with that of mAb 7.30 (cluster 4). It displays a stronger reaction with Daudi than with U937 cells. The epitope is located in domain 2 of FcgRIIa. Its Fab'2 fragments block immune complex binding. CD32 (FcgRII) is a type 1 transmembrane glycoprotein that mediates several functions including phagocytosis, cytotoxicity, and immunomodulation as well as platelet aggregation. Three genes (A, B, and C) encode CD32 and at least 6 isoforms are generated via alternative mRNA splicing, i.e., IIa1, IIa2, IIb1, IIb2, IIb3 and IIc. Monocytes/macrophages, placental trophoblasts and endothelial cells express all isoforms. In addition, the IIb isoform is expressed by B cells, and the IIa isoform by platelets, granulocytes and, weakly, by B cells. NK cells and neutrophils express Isoform IIc. CD32 binds weakly to the Fc region of monomeric IgG but more strongly to IgG aggregates and immune complexes.

Immunogen: K562 and FcgRII+L cells were used as the immunogen for the CD32 antibody.

Storage: Store the CD32 antibody at 2-8oC (with azide) or aliquot and store at -20 °C or colder (without azide).