Description
CD31 Antibody / PECAM-1 | V8788-20UG | Gentaur US, UK & Europe Disrtribition
Family: Primary antibody
Formulation: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Format: Purified
Clone: r6439
Host: Mouse
Clonality: Recombinant Mouse Monoclonal
Isotype: Mouse IgG1, kappa
Species Reactivity: Human
Application: FACS, WB, IHC-P
Application Details: Flow cytometry: 0.5-1ug/million cells,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Application Note: Optimal dilution of the CD31 antibody should be determined by the researcher.
Purity: Protein A/G affinity
Description: CD31 (PECAM-1) is a transmembrane glycoprotein member of the immunoglobulin supergene family of adhesion molecules. CD31 is expressed by stem cells of the hematopoietic system and is primarily used to identify and concentrate these cells for experimental studies as well as for bone marrow transplantation. Anti-CD31 has shown to be highly specific and sensitive for vascular endothelial cells. Staining of nonvascular tumors (excluding hematopoietic neoplasms) is rare. CD31 MAb reacts with normal, benign, and malignant endothelial cells which make up blood vessel lining. The level of CD31 expression can help to determine the degree of tumor angiogenesis, and a high level of CD31 expression may imply a rapidly growing tumor and potentially a predictor of tumor recurrence.
Immunogen: A portion of amino acids 28-330 was used as the immunogen for the CD31 antibody.
Storage: Aliquot the CD31 antibody and store frozen at -20 °C or colder. Avoid repeated freeze-thaw cycles.
Localization: Cell surface and cytoplasm of endothelial cells
