Description
CD3 epsilon Antibody | V7557-20UG | Gentaur US, UK & Europe Disrtribition
Family: Primary antibody
Formulation: 0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Format: Purified
Clone: C3e/3125R
Host: Rabbit
Clonality: Recombinant Rabbit Monoclonal
Isotype: Rabbit IgG
Species Reactivity: Human
Application: ELISA, WB, IHC-P
Application Details: ELISA (order BSA-free format for coating),Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Application Note: Optimal dilution of the recombinant CD3e antibody should be determined by the researcher.
Purity: Protein A affinity chromatography
Description: Recognizes the epsilon-chain of CD3, which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.
Immunogen: A recombinant human partial protein (amino acids 23-119) was used as the immunogen for the recombinant CD3e antibody.
Storage: Store the recombinant CD3e antibody at 2-8oC (with azide) or aliquot and store at -20 °C or colder (without azide).
Localization: Cell surface, cytoplasmic
