CD274 Polyclonal Antibody | E-AB-70312

CD274 Polyclonal Antibody | E-AB-70312 | Gentaur US, UK & Europe Disrtribition

Reactivity: Human, Mouse, Rat

Host: Rabbit

Isotype: IgG

Application: WB, IHC

Research Area: Cancer, Immunology

Background: Programmed cell death ligand 1(CD274, or B7-H1, PD-L1), is the first member of B7 family to be discovered. B7 family molecules are type I transmembrane proteins belonging to the immunoglobulin superfamily. In concert with their CD28 family receptors, the B7s are key regulators of the adaptive immune response. CD274 is suggested a negative regulator of T and B cell, and play important role in mediating tolerance of lymphocytes to self-antigens. It also involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. PD-L1 is a 290 aa transmembrane protein with a calculated molecular weight of 33 kDa, the apparent molecular weight has been reported as 45-70 kDa, suggesting probable glycosylation .

Lead Time: 7~10 days

Concentration: 0.9 mg/mL

Storage: Store at -20°C. Avoid freeze / thaw cycles.

Immunogen: KLH conjugated Synthetic peptide corresponding to Mouse PD-L1

Buffer: PBS with 0.02% sodium azide, 1% BSA and 50% glycerol, pH7.4

Purification: Affinity purification

Dilution: WB 1:500-1:2000, IHC 1:200-1:600

Conjugation: Unconjugated

Calculated Molecular Weight: 33 kDa

Observed Molecular Weight: 50 kDa

Clonality: Polyclonal