CD27 Antibody | V3558-100UG

CD27 Antibody | V3558-100UG | Gentaur US, UK & Europe Disrtribition

Family: Primary antibody

Formulation: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Format: Purified

Clone: rLPFS2/1611

Host: Mouse

Clonality: Recombinant Mouse Monoclonal

Isotype: Mouse IgG1, kappa

Species Reactivity: Human

Application: FACS, IHC-P

Application Details: Flow cytometry: 1-2ug/million cells,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT

Application Note: Optimal dilution of the recombinant CD27 antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Purity: Protein G affinity chromatography

Description: Recognizes a protein of a disulfide-linked 120kDa dimer, identified as CD27. It is expressed on the majority of peripheral T cells, medullary thymocytes, memory-type B cells, and natural killer cells. It is a transmembrane phosphoglycoprotein that belongs to the tumor necrosis factor receptor (TNFR) superfamily. CD27 binds to its ligand CD70, a member of the TNF family, and induces T-cell co-stimulation and B-cell activation. It also interacts with TRAFs and is involved in activation of NFkB and SAPK/JNK and induces apoptosis.

Immunogen: Human CD27 protein was used as the immunogen for the recombinant CD27 antibody.

Storage: Store the recombinant CD27 antibody at 2-8oC (with azide) or aliquot and store at -20 °C or colder (without azide).

Localization: Cell surface