Description
CD21 Antibody | V7754-20UG | Gentaur US, UK & Europe Disrtribition
Family: Primary antibody
Formulation: 0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Format: Purified
Clone: CR2/3124R
Host: Rabbit
Clonality: Recombinant Rabbit Monoclonal
Isotype: Rabbit IgG
Species Reactivity: Human
Application: FACS, IF, WB, IHC-P
Application Details: Flow cytometry: 1-2ug/million cells in 0.1ml,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Application Note: Optimal dilution of the antibody should be determined by the researcher.
Purity: Protein A affinity chromatography
Description: Recognizes a protein of 140kDa, which is identified as the complement receptor 2 (CR2) or CD21. This protein is expressed strongly on mature B cells, follicular dendritic cells and weakly on immature thymocytes and T lymphocytes. In B-cell ontogeny, CD21 appears after the pre-B-stage, is maintained during peripheral B-cell development and is lost upon terminal differentiation into plasma cells. CD21 expression is also gradually lost after stimulation of B cells in vitro. CD21 functions as receptor for C3d, C3dg and iC3b Complement components, for EBV and for IFNalpha. CD21 binds to CD23 and associates with CD19, CD81 and Leu13 to form a large signal-transduction complex involved in B cell activation.
Immunogen: A partial protein corresponding to amino acids 142-240 was used as the immunogen for the recombinant CD21 antibody.
Storage: Store the recombinant CD21 antibody at 2-8oC (with azide) or aliquot and store at -20 °C or colder (without azide).
Localization: Cell surface
