CCRF-CEM [CCRF CEM] Cell Line | CL-0058

CCRF-CEM [CCRF CEM] Cell Line | CL-0058 | Gentaur US, UK & Europe Disrtribition

Media: CM-0058

Organism: Homo sapiens, Human

Application: Transfection host.

Background: CCRF-CEM cells were derived from the peripheral blood buffy coat of a child(CEM) with acute lymphoblastic leukemia who had originally presented with lymphosarcoma. Do not centrifuge after thawing.

Age: 4 years juvenile

Tissue: Peripheral blood

Morphology: Lymphoblast

Growth Properties: Suspension

Doubling Time: N/A

Biosafty: 1

Medium: RPMI-1640(PM150110)+10% FBS+1% P/S(PB180120)

Subculturing: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at the subcultivation ratio of 1:3-1:5.

Ratio: 1:2 to 1:4

Renewal: Every 2 to 3 days

Cryopreservation: Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase

Culture Conditions: Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃

Effects: Yes, in syrian hamsters.

Duration: N/A

Lead Time: 3-5 days