Cas9-Expressing MDA-MB-231 Cell Line (High or Low Expression)-78150-L | 78150-L

Cas9-Expressing MDA-MB-231 Cell Line (High or Low Expression)-78150-L | 78150-L | Gentaur US, UK & Europe Disrtribition

Category: CRISPR/Cells

Application: 1. Quickly generating knock-out cell pools or cell lines in MDA-MB-231 cell pools.
2. Implementing sgRNA screens in Cas9 expressing MDA-MB-231 cell pools.

Background: Cas9 (Streptococcus pyogenes CRISPR associated protein 9) is an endonuclease enzyme that, when recruited to a specific DNA sequence by the sgRNA (single guide RNA), introduces a double stranded break into the DNA. This double stranded break is repaired in mammalian cells either through Non-Homologous End Joining or Homologous Recombination. Non-Homologous End Joining often results in the deletion or insertion of several base pairs at the cut site, which, when resulting in a frameshift, causes the functional inactivation of the targeted gene. Cas9-expressing MDA-MB-231 cells can be transduced or electroporated with sgRNA targeting a gene of interest to quickly generate knock-out cell pools or cell lines.

Description: This cell line is a clonal derivative from the Cas9-Expressing MDA-MB-231 Cell Pool (BPS Bioscience, #78069). It was generated by limited limiting dilution of the original pool and isolation of individual clones, which were screened based on Cas9 expression to obtain a low- or high-expressing cell line. The expressed Cas9 protein includes a C-terminal FLAG tag.

Product Type: Cell Line

Shippement Condition: -80°C