Description
Cas9-Expressing A549 cell pool | 78072 | Gentaur US, UK & Europe Disrtribition
Category: CRISPR/Cells
Application: 1. Quickly generating knock-out cell pools or cell lines in A549 cells.
2. Implementing sgRNA screens in Cas9-expressing A549 cells.
Background: N/A
Description: Cas9 (Streptococcus pyogenes CRISPR associated protein 9) is an endonuclease enzyme that, when recruited to a specific DNA sequence by the sgRNA (single guide RNA), introduces a double stranded break into the DNA. This double stranded break is repaired in mammalian cells either through Non-Homologous End Joining or Homologous Recombination. Non-Homologous End Joining often results in the deletion or insertion of several base pairs at the cut site, which, when resulting in a frameshift, causes the functional inactivation of the targeted gene. Cas9-expressing A549 cells can be transduced or electroporated with sgRNA targeting a gene of interest to quickly generate knock-out cell pools or cell lines.
Product Type: Cell Line
Shippement Condition: -80°C
