Description
Capan-2 Cell Line | CL-0709 | Gentaur US, UK & Europe Disrtribition
Media: CM-0709
Organism: Homo sapiens, Human
Application: N/A
Background: N/A
Age: 56 years
Tissue: Pancreas
Morphology: Polygonal
Growth Properties: Adherent
Doubling Time: 96 hours (PubMed=3019537); 45-60 hours (CLS); ~50-70 hours (DSMZ).
Biosafty: 1
Medium: McCoy's 5A(PM150710)+10%FBS+1%P/S(PB180120)
Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Ratio: 1:2 to 1:4
Renewal: 2 to 3 times per week
Cryopreservation: Freeze Medium: 55% Basal Medium+40% FBS+5% DMSOStorage temperature: Liquid nitrogen vapor phase
Culture Conditions: Atmosphere: Air, 95%; CO2, 5%Temperature: 37℃
Effects: N/A
Duration: N/A
Lead Time: 3-5 days
