Description
Capan-1 Cell Line | CL-0708 | Gentaur US, UK & Europe Disrtribition
Media: CM-0708
Organism: Homo sapiens, Human
Application: N/A
Background: hypotriploid with abnormalities including dicentrics, breaks, acrocentric fragments, large submetacentric and subtelocentric chromosomes plus minute marker
Age: 40 years
Tissue: Pancreas
Morphology: Epithelial
Growth Properties: Adherent
Doubling Time: 60-80 hours (CLS); ~50-100 hours (DSMZ); ~49 hours (PBCF).
Biosafty: 1
Medium: IMDM(PM150510)+20%FBS+1%P/S(PB180120)
Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Ratio: 1:2 to 1:4
Renewal: every 2 to 3 days
Cryopreservation: Freeze Medium: 55% Basal Medium+40% FBS+5% DMSOStorage temperature: Liquid nitrogen vapor phase
Culture Conditions: Atmosphere: Air, 95%; CO2, 5%Temperature: 37℃
Effects: N/A
Duration: N/A
Lead Time: 3-5 days
