Canine CD73 / NT5E Protein, His Tag (MALS verified) (active enzyme) | CD3-C52H5-100ug

Canine CD73 / NT5E Protein, His Tag (MALS verified) (active enzyme) | CD3-C52H5-100ug| Gentaur Distribution US, UK & Europe

5'-nucleotidase (5'-NT), also known as ecto-5'-nucleotidase or CD73 (cluster of differentiation 73), is an enzyme that is encoded by the NT5E gene. CD73 commonly serves to convert AMP to adenosine. Ecto-5-prime-nucleotidase (5-prime-ribonucleotide phosphohydrolase) catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate. Rare allelic variants are associated with a syndrome of adult-onset calcification of joints and arteries (CALJA) affecting the iliac, femoral, and tibial arteries reducing circulation in the legs and the joints of the hands and feet causing pain.

Source: Canine CD73, His Tag (CD3-C52H5) is expressed from human 293 cells (HEK293). It contains AA Trp 27 - Ser 549 (Accession # XP_038540011.1).

Format: Powder.

Tag: C-10×His.

Expression System: HEK293.

Expression Region: Trp 27 - Ser 549.

Conjugate: Unconjugated.

Molecular Weight: 59.8 kDa.

Characteristics: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 59.8 kDa. The protein migrates as 65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Purity: 95%.

Buffer: 20 mM Tris, 120mM NaCl, pH7.5.

Storage Conditions: -20℃.

Shipping Conditions: RT