Calu-1 cell line | CL-0053

Calu-1 cell line | CL-0053 | Gentaur US, UK & Europe Disrtribition

Media: CM-0053

Organism: Homo sapiens, Human

Application: N/A

Background: This cell line is derived from an epidermoid carcinoma of the lung in a 47-year-old Caucasian male. The line can form epidermoid carcinomas in nude mice and tumours in steroid treated hamsters. Ultrastructure includes numerous microvilli, prominent rough endoplasmic reticulum, lysosomes, lipid inclusions and no virus particles. This cell line contains the ras (K-ras) oncogene.

Age: male, 47 years

Tissue: Lung; Derived From Metastatic Site: Pleura

Morphology: Epithelial

Growth Properties: Adherent

Doubling Time: ~37 hours

Biosafty: 1

Medium: McCoy's 5A(PM150710)+10% FBS+1% P/S(PB180120)

Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.

Ratio: 1:2 to 1:4

Renewal: 2 to 3 times per week

Cryopreservation: Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase

Culture Conditions: Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃

Effects: N/A

Duration: N/A

Lead Time: 3-5 days