Caldesmon Antibody / CALD1 / CAD (HMW) | V9402SAF-100UG

Caldesmon Antibody / CALD1 / CAD (HMW) | V9402SAF-100UG | Gentaur US, UK & Europe Disrtribition

Family: Primary antibody

Formulation: 1 mg/ml in 1X PBS; BSA free, sodium azide free

Format: Purified

Clone: rCALD1/7266

Host: Mouse

Clonality: Recombinant Mouse Monoclonal

Isotype: Mouse IgG1, kappa

Species Reactivity: Human

Application: IHC-P

Application Details: Immunohistochemistry (FFPE): 1-2ug/ml

Application Note: Optimal dilution of the recombinant CALD1 antibody should be determined by the researcher.

Purity: Protein A/G affinity

Description: Recognizes a protein of 150kDa, which is identified as the high molecular weight variant of Caldesmon. Two closely related variants of human caldesmon have been identified which are different in their electrophoretic mobility and cellular distribution. The h-caldesmon variant (120-150kDa) is predominantly expressed in smooth muscle whereas l-caldesmon (70-80kDa) is found in non- muscle tissue and cells. Neither of the two variants has been detected in skeletal muscle. This MAb recognizes only the 150kDa variant (h-caldesmon) in Western blots of human aortic media extracts and is unreactive with fibroblast extracts from cultivated human foreskin. Caldesmon is a developmentally regulated protein involved in smooth muscle and non-muscle contraction.

Immunogen: Recombinant human full-length CALD1 protein was used as the immunogen for the recombinant CALD1 antibody.

Storage: Aliquot the recombinant CALD1 antibody and store frozen at -20 °C or colder. Avoid repeated freeze-thaw cycles.

Localization: Cytoplasm