Description
Caldesmon Antibody / CALD1 / CAD (HMW) | V8878-100UG | Gentaur US, UK & Europe Disrtribition
Family: Primary antibody
Formulation: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Format: Purified
Clone: rCALD1/820
Host: Mouse
Clonality: Recombinant Mouse Monoclonal
Isotype: Mouse IgG1, kappa
Species Reactivity: Human
Application: WB, IHC-P
Application Details: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 0.25-0.5ug/ml
Application Note: Optimal dilution of the recombinant Caldesmon antibody should be determined by the researcher.
Purity: Protein A/G affinity
Description: Recognizes a protein of 150kDa, which is identified as the high molecular weight variant of Caldesmon. Two closely related variants of human caldesmon have been identified which are different in their electrophoretic mobility and cellular distribution. The h-caldesmon variant (120-150kDa) is predominantly expressed in smooth muscle whereas l-caldesmon (70-80kDa) is found in non- muscle tissue and cells. Neither of the two variants has been detected in skeletal muscle. This MAb recognizes only the 150kDa variant (h-caldesmon) in Western blots of human aortic media extracts and is unreactive with fibroblast extracts from cultivated human foreskin. Caldesmon is a developmentally regulated protein involved in smooth muscle and non-muscle contraction.
Immunogen: Recombinant full-length human CALD1 protein was used as the immunogen for the recombinant Caldesmon antibody.
Storage: Aliquot the recombinant Caldesmon antibody and store frozen at -20 °C or colder. Avoid repeated freeze-thaw cycles.
Localization: Cytoplasmic
