Description
CAL 27 Cell Line | CL-0265 | Gentaur US, UK & Europe Disrtribition
Media: CM-0265
Organism: Homo sapiens, Human
Application: N/A
Background: CAL 27 cells are epithelial, polygonal with a highly granular cytoplasm. Immunocytochemical studies show strong positive staining with anti keratin antibodies. The cells do not grow well in semi-solid medium. Marked inhibition of Thymidine incorporation was observed in the presence of VP16(etoposide), CCNU(1-[2-chloroethyl]-3-cyclohexyl-1-nitrosourea), VM26(teniposide), ADM(adriamycin), CPA(cyclophosphamide), and MTX(Methotrexate). CAL 27 cells were resistant to treatment with VDS(vindesine sulfate), CDP(cis-platinum) or ACTD(actinomycin D). A culture submitted to the ATCC in December 1993 was found to be contaminated with mycoplasma. Progeny were cured by a 21-day treatment with BM Cycline.
Age: 56 years
Tissue: Tongue
Morphology: Epithelial
Growth Properties: Adherent
Doubling Time: N/A
Biosafty: 1
Medium: DMEM(PM150210)+10% FBS+1% P/S(PB180120)
Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Ratio: 1:2 to 1:4
Renewal: Every 2 to 3 days
Cryopreservation: Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture Conditions: Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
Effects: Yes, solid tumors developed within 6 weeks in nude mice inoculated with 2×10^6 cells subcutaneously.
Duration: N/A
Lead Time: 3-5 days
