Caki-1 Cell Line | CL-0052

Caki-1 Cell Line | CL-0052 | Gentaur US, UK & Europe Disrtribition

Media: CM-0052

Organism: Homo sapiens, Human

Application: Transfection host.

Background: Ultrastructural features include many microvilli, few filaments, many small mitochondria, well developed Golgi and ER, many lipid droplets and multilaminate bodies, secondary lysosomes, no virus particles.

Age: 49 years

Tissue: Kidney, derived from metastatic site: skin

Morphology: Epithelial

Growth Properties: Adherent

Doubling Time: N/A

Biosafty: 1

Medium: McCoy's 5A(PM150710)+10% FBS+1% P/S(PB180120)

Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.

Ratio: 1:2 to 1:4

Renewal: Every 2 to 3 days

Cryopreservation: Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase

Culture Conditions: Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃

Effects: Yes, in nude mice forms clear cell carcinoma in nude mice consistent with renal primary; also forms tumors in steroid treated hamsters.

Duration: N/A

Lead Time: 3-5 days