Ca Ski Cell Line | CL-0048

Ca Ski Cell Line | CL-0048 | Gentaur US, UK & Europe Disrtribition

Media: CM-0048

Organism: Homo sapiens, Human

Application: N/A

Background: The cells are reported to contain an integrated human papillomavirus type 16 genome(HPV-16, about 600 copies per cell) as well as sequences related to HPV-18. ATCC has confirmed this cell line is positive for the presence of human papilloma virus(HPV) viral sequences via PCR.

Age: 40 years adult

Tissue: Cervix, derived from metastatic site: small intestine

Morphology: Epithelial

Growth Properties: Adherent

Doubling Time: N/A

Biosafty: 2

Medium: RPMI-1640(PM150110)+10% FBS+1% P/S(PB180120)

Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.

Ratio: 1:2 to 1:4

Renewal: Every 2 to 3 days

Cryopreservation: Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase

Culture Conditions: Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃

Effects: N/A

Duration: N/A

Lead Time: 3-5 days