C127 [C-127] Cell Line | CL-0043

C127 [C-127] Cell Line | CL-0043 | Gentaur US, UK & Europe Disrtribition

Media: CM-0043

Organism: Mus musculus, Mouse

Application: Useful for visualization of sarcoma virus-induced foci and for an in vitro quantitative assay for bovine papilloma virus. Host for transfection with plasmids containing bovine papilloma virus DNA. C127I cells are suitable hosts for transformation with bov

Background: Tested and found negative for ectromelia virus(mousepox).

Age: N/A

Tissue: Mammary gland

Morphology: Epithelial

Growth Properties: Adherent

Doubling Time: N/A

Biosafty: 1

Medium: DMEM(PM150210)+10% FBS+1% P/S(PB180120)

Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.

Ratio: 1:2 to 1:4

Renewal: Every 2 to 3 days

Cryopreservation: Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase

Culture Conditions: Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃

Effects: N/A

Duration: N/A

Lead Time: 3-5 days