BT-20 Cell Line | CL-0324

BT-20 Cell Line | CL-0324 | Gentaur US, UK & Europe Disrtribition

Media: CM-0324

Organism: Homo sapiens, Human

Application: N/A

Background: The cells express the WNT3 and the WNT7B oncogenes . Growth of BT-20 cells is inhibited by tumor necrosis factor alpha (TNF alpha). BT-20 cells are negative for estrogen receptor, but do express an estrogen receptor mRNA that has deletion of exon 5.

Age: 74 years

Tissue: Mammary gland/breast

Morphology: Epithelial

Growth Properties: Adherent

Doubling Time: ~70 hours

Biosafty: 1

Medium: MEM (PM150410)+10% FBS+1% P/S (PB180120)

Subculturing: N/A

Ratio: 1:2 to 1:4

Renewal: every 2 to 3 days

Cryopreservation: Freeze Medium: 55% Basal Medium+40% FBS+21% DMSO Storage temperature: Liquid nitrogen vapor phase

Culture Conditions: Atmosphere: Air, 95%; CO2, 5%; Temperature: 37℃

Effects: Yes, in nude mice(The cells form grade II adenocarcinomas.).

Duration: N/A

Lead Time: 3-5 days