BS-C-1 cell line | CL-0039

BS-C-1 cell line | CL-0039 | Gentaur US, UK & Europe Disrtribition

Media: CM-0039

Organism: Cercopithecus aethiops

Application: N/A

Background: BS-C-1 is a kidney epithelial cell line isolated in 1961 from monkey (Cercopithecus aethiops). The cells are sensitive to Poliovirus type 1, SV40 virus and Vesicular stomatitis virus and can develop characteristic cytopathic effects after infection. This cell line is a suitable tool for virus detection.

Age: N/A

Tissue: Kidney

Morphology: Epithelial

Growth Properties: Adherent

Doubling Time: ~72 hours

Biosafty: 1

Medium: MEM(PM150410)+10% FBS+1% P/S(PB180120)

Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.

Ratio: 1:3 to 1:4

Renewal: 2 to 3 times per week

Cryopreservation: Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase

Culture Conditions: Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃

Effects: N/A

Duration: N/A

Lead Time: 3-5 days