Description
Bromodeoxyuridine / BrdU Antibody | V3928-20UG | Gentaur US, UK & Europe Disrtribition
Family: Primary antibody
Formulation: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Format: Purified
Clone: BRD2888R
Host: Rabbit
Clonality: Recombinant Rabbit Monoclonal
Isotype: Rabbit IgG
Species Reactivity: All species
Application: IHC-P
Application Details: Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
Application Note: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Bromodeoxyuridine / BrdU antibody to be titered up or down for optimal performance.
1. For staining of formalin-fixed tissues, incubate sections in 4N HCl for 30 minutes at RT followed by digestion with trypsin at 1mg/ml PBS, 10 min at 37oC.
Purity: Protein A affinity chromatography
Description: This antibody reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to Bromodeoxyuridine / BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.
Immunogen: BrdU / Bromodeoxyuridine conjugated to KLH was used as the immunogen for this antibody.
Storage: Store the BrdU antibody at 2-8oC (with azide) or aliquot and store at -20 °C or colder (without azide).
Localization: Nuclear
